Data/hora: 10/06/2024 08:56:56

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)	
Autores: 	Presti,Flavia T.; Meyer,Janaína; Antas,Paulo T.Z.; Guedes,Neiva M.R.; Miyaki,Cristina Y.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Molted feathers; Blood sample; DNA; PCR product; Parrot.	
Resumo: 	Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572013005000001	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.36 n.1 2013	
Fonte: 	info:eu-repo/semantics/openAccess