Data/hora: 09/06/2024 03:34:06

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Analysis of the population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms	
Autores: 	Rincón,Gonzalo; Armstrong,Eileen; Postiglioni,Alicia.	
Data: 	2006-01-01	
Ano: 	2006	
Palavras-chave: 	DGAT1 gene; Milk protein; SNPs; Uruguayan Creole cattle.	
Resumo: 	The ancestors of Uruguayan Creole cattle were introduced by the Spanish conquerors in the XVII century, following which the population grew extensively and became semi-feral before the introduction of selected breeds. Today the Uruguayan Creole cattle genetic reserve consists of 575 animals. We used the tetra primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to analyze the kappa-casein, beta-casein, alphaS1-casein and alpha-lactoalbumin gene polymorphisms and restriction fragment length polymorphism PCR (RFLP-PCR) for the beta-lactoglobulin and the acylCoA:diacyl glycerol acyltransferase 1 (DGAT1) genes. The kappa-casein and beta-lactoglobulin genes presented very similar A and B allele frequencies, while the alphas1-casein and alpha-lactoalbumin gene B alleles showed much higher frequencies than the corresponding A alleles. The beta-casein B allele was not found in the population sampled. There was a very high frequency of the DGAT1 gene A allele which is associated with low milk fat content and high milk yield. All loci were in Hardy-Weinberg equilibrium and the level of heterozygosity agreed with the high genetic diversity observed in a previous analysis of this population. Preservation of the allelic richness observed in the Uruguayan Creole cattle should be considered for future dairy management and livestock genetic improvement. The results also emphasize the value of the tetra primers ARMS-PCR technique as a rapid, easy and economical way of genotyping cattle breeds for milk gene single nucleotide polymorphisms.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300016	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572006000300016	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.29 n.3 2006	
Fonte: 	info:eu-repo/semantics/openAccess