Data/hora: 09/06/2024 03:38:36

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress
Autores: | Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response.
Resumo: | CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and proline-related synthase. Some sequences could not be classified by using FunCat and remained with an unknown function. A large number of sequences presented high similarities to annotated genes involved with cell energy, protein synthesis and cellular transport, suggesting that Rangpur lime may sustain active cell growth under stressed condition. The presence of membrane transporters and cell signaling components could be an indication of a coordinated morphological adaptation and biochemical response during drought, helping to explain the higher tolerance of this rootstock to water stress.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000500019
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.3 suppl.0 2007
Fonte: | info:eu-repo/semantics/openAccess