Data/hora: 08/06/2024 15:28:50

Provedor de dados: | 74
País: | Brazil
Título: | High-throughput nucleotide sequence analysis of diverse bacterial communities in leachates of decomposing pig carcasses
Autores: | Yang,Seung Hak; Lim,Joung Soo; Khan,Modabber Ahmed; Kim,Bong Soo; Choi,Dong Yoon; Lee,Eun Young; Ahn,Hee Kwon.
Data: | 2015-09-01
Ano: | 2015
Palavras-chave: | Pig; Decomposition; Leachate; Bacterial community; Pyrosequencing.
Resumo: | The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from the decomposition of pig carcasses. We acquired 51,230 reads from six different samples (1, 2, 3, 4, 6 and 14 week-old carcasses) and found that sequences representing the phylum Firmicutes predominated. The diversity of bacterial 16S rRNA gene sequences in the leachate was the highest at 6 weeks, in contrast to those at 2 and 14 weeks. The relative abundance of Firmicutes was reduced, while the proportion of Bacteroidetes and Proteobacteria increased from 3–6 weeks. The representation of phyla was restored after 14 weeks. However, the community structures between the samples taken at 1–2 and 14 weeks differed at the bacterial classification level. The trend in pH was similar to the changes seen in bacterial communities, indicating that the pH of the leachate could be related to the shift in the microbial community. The results indicate that the composition of bacterial communities in leachates of decomposing pig carcasses shifted continuously during the study period and might be influenced by the burial site.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300373
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-475738320140252
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.38 n.3 2015
Fonte: | info:eu-repo/semantics/openAccess