Data/hora: 10/06/2024 07:05:14

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species	
Autores: 	Wasko,Adriane P.; Severino,Fábio E.; Presti,Flávia T.; Poletto,Andréia B.; Martins,Cesar.	
Data: 	2007-01-01	
Ano: 	2007	
Palavras-chave: 	Actin gene; Amino acid residues; Gene structure; Nile tilapia; Nucleotide sequence; Oreochromis niloticus.	
Resumo: 	An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572007000600010	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.30 n.4 2007	
Fonte: 	info:eu-repo/semantics/openAccess