Data/hora: 02/06/2024 09:31:32

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Differential detection of transposable elements between Saccharum species	
Autores: 	Souza,Marislane Carvalho Paz de; Silva,Jéssica Naiana; Almeida,Cícero.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Sugarcane; Dot-blot hybridization; LTR retrotransposons; Repetitive DNA.	
Resumo: 	Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S. officinarum, 10-15% from S. spontaneum, and 5-10% are possible recombinants between the two genomes. The aim of this study was to analyze the repetition of DNA sequences in S. officinarum and S. spontaneum. For this purpose, genomic DNA from S. officinarum was digested with restriction enzymes and the fragments cloned. Sixty-eight fragments, approximately 500 bp, were cloned, sequenced and had their identity analyzed in NCBI, and in the rice, maize, and sorghum genome databases using BLAST. Twelve clones containing partial transposable elements, one single-copy control, one DNA repetitive clone control and two genome controls were analyzed by DNA hybridization on membrane, using genomic probes from S. officinarum and S. spontaneum. The hybridization experiment revealed that six TEs had a similar repetitive DNA pattern in the genomes of S. officinarum and S. spontaneum, while six TEs were more abundant in the genome of S. officinarum. We concluded that the species S. officinarum and S. spontaneum have differential accumulation LTR retrotransposon families, suggesting distinct insertion or modification patterns.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300016	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572013005000030	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.36 n.3 2013	
Fonte: 	info:eu-repo/semantics/openAccess