Data/hora: 04/06/2024 04:22:20

Provedor de dados: | 74
País: | Brazil
Título: | Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays
Autores: | Leite-Silva,Cleide; Gusmão,Cássia Lima Silva; Takahashi,Catarina Satie.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Antigenotoxicity; Chromosomal aberrations; Comet assay; Doxorubicin; Fucus vesiculosus.
Resumo: | The brown seaweed Fucus vesiculosus (Fucales, Fucaceae) was screened for its protective activity using doxorubicin-induced DNA damage in human lymphocytes. In this study, we assessed the genotoxic and antigenotoxic potential of three different concentrations (0.25, 0.5 and 1.0 mg mL-1) of F. vesiculosus aqueous extract using the chromosome aberration and Comet assays. Treatment of human lymphocyte cultures with 0.25, 0.5 and 1.0 mg mL-1 F. vesiculosus aqueous extract had no effect on the chromosome aberration frequency or on the extent of DNA damage detected by the Comet assay. The antigenotoxic effects of the extract were tested in human lymphocyte cultures treated with 15 µg mL-1 of doxorubicin, either alone or combined with the different concentrations of the extract, which was added to the cultures before, simultaneously with or after the doxorubicin. Only when lymphocytes were pre-treated with extract there was a reduction in doxorubicin-induced chromosome aberrations and DNA damage as detected by the Comet assay. These results demonstrate that F. vesiculosus aqueous extract is not genotoxic in cultured human lymphocytes and indicate that when added to lymphocyte cultures before doxorubicin it has antigenotoxic activity against doxorubicin-induced DNA damage.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100019
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000100019
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess