Data/hora: 09/06/2024 02:50:47

Provedor de dados: | 74
País: | Brazil
Título: | Transcriptome sequencing analysis of alfalfa reveals CBF genes potentially playing important roles in response to freezing stress
Autores: | Shu,Yongjun; Li,Wei; Zhao,Jinyue; Zhang,Sijia; Xu,Hanyun; Liu,Ying; Guo,Changhong.
Data: | 2017-12-01
Ano: | 2017
Palavras-chave: | Medicago sativa L.; RNA-seq; Freezing tolerance; C-repeat binding factors (CBF).
Resumo: | Abstract Alfalfa (Medicago sativa L.) is an important perennial forage, with high nutritional value, which is widely grown in the world. Because of low freezing tolerance, its distribution and production are threatened and limited by winter weather. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed transcriptome sequencing analysis under cold (4 °C) and freezing (-8 °C) stresses. More than 66 million reads were generated, and we identified 5767 transcripts differentially expressed in response to cold and/or freezing stresses. These results showed that these genes were mainly classified as response to stress, transcription regulation, hormone signaling pathway, antioxidant, nodule morphogenesis, etc., implying their important roles in response to cold and freezing stresses. Furthermore, nine CBF transcripts differentially expressed were homologous to CBF genes of Mt-FTQTL6 site, conferring freezing tolerance in M. truncatula, which indicated that a genetic mechanism controlling freezing tolerance was conservative between M. truncatula and M. sativa. In summary, this transcriptome dataset highlighted the gene regulation response to cold and/or freezing stresses in alfalfa, which provides a valuable resource for future identification and functional analysis of candidate genes in determining freezing tolerance.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500824
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2017-0053
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.40 n.4 2017
Fonte: | info:eu-repo/semantics/openAccess