Data/hora: 02/06/2024 06:03:17

Provedor de dados: | 74
País: | Brazil
Título: | Karyotype, C-and fluorescence banding pattern, NOR location and FISH study of five Scarabaeidae (Coleoptera) species
Autores: | Bione,Edgar; Moura,Rita de Cássia de; Carvalho,Reginaldo de; Souza,Maria José de.
Data: | 2005-09-01
Ano: | 2005
Palavras-chave: | Karyotype; Constitutive heterochromatin; NORs; RDNA sequences.
Resumo: | Meiotic chromosomes obtained from members of the coleopteran subfamilies Rutelinae and Dynastinae were studied using standard and silver nitrate staining, C-banding, base-specific fluorochromes and fluorescent in situ hybridization (FISH). The study presents detailed karyotipic descripitions of three Rutelinae species (Geniates borelli, Macraspis festiva and Pelidnota pallidipennis), and two Dynastinae species (Lygirus ebenus and Strategus surinamensis hirtus) with special emphasis on the distribution and variability of constitutive heterochromatin and the nucleolar organizer region (NOR). We found that for G. borelli, P. pallidipennis, L. ebenus and S. s hirtus the karyotype was 2n = 20 (9II + Xy p), with G. borelli, P. pallidipennis and L. ebenus showed meta-submetacentric chromosomes which gradually decreased in size. For Macraspis festiva the karyotype was 2n = 18 (8II + Xy p). In L. ebenus we found that the NOR was located on an autosome, but in the other four species it occurred on the sex bivalents. In all five species the constitutive heterochromatin (CH) was predominantly pericentromeric while the X chromosomes were almost completely heterochomatic, although CMA3/DA/DAPI staining showed intra and interspecific variation in the bright fluorescence of the constitutive heterochromatin. The FISH technique showed rDNA sites on the X chromosome of the Rutelinae species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300006
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572005000300006
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.28 n.3 2005
Fonte: | info:eu-repo/semantics/openAccess