Data/hora: 10/06/2024 05:35:15

Provedor de dados: | 74
País: | Brazil
Título: | Autonomous growth of BALB/MK keratinocytes transfected   with a retroviral vector carrying the human epidermal growth factor gene
Autores: | Choudhuri,Jomuna V.; Mathor,Monica B.; Silva,Flávia H.; Han,Sang W.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | BALB/MK keratinocytes; Epidermal growth factor; Retroviral vector.
Resumo: | Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. In this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. The transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time- and cost-efficient means of culturing keratinocytes and yields cells that may be useful for skin grafting.
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500003
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572008005000009
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.31 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess