Data/hora: 09/06/2024 13:32:51

Provedor de dados: | 74
País: | Brazil
Título: | Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Autores: | Nora,Luísa Czamanski; Gonçales,Relber Aguiar; Martins-Santana,Leonardo; Ferreira,Beatriz Henriques; Rodrigues,Fernando; Silva-Rocha,Rafael.
Data: | 2019-06-01
Ano: | 2019
Palavras-chave: | Synthetic biology; Fungi; Agrobacterium tumefaciens; Transformation; Vectors.
Resumo: | Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation: minimalist, synthetic, modular, and available in four different versions, and these can still be readily modified using a few primers and few cloning steps.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300395
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2018-0221
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.42 n.2 2019
Fonte: | info:eu-repo/semantics/openAccess