Data/hora: 10/06/2024 17:07:44

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology
Autores: | Cassel,Ana Paula Rebelo; Barcellos,Regina Bones; Silva,Cláudia Maria Dornelles da; Almeida,Sabrina Esteves de Matos; Rossetti,Maria Lucia Rosa.
Data: | 2014-06-01
Ano: | 2014
Palavras-chave: | Human papillomavirus; Micronucleus; Cytology; High-risk HPV; Southern Brazil.
Resumo: | The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples (102) may be of great value in predicting the absence of HPV. The mean age of HPV-DNA positive women (34.2 ± 12.6) was significantly lower than the mean age of HPV-DNA negative women (43.9 ± 13.7) (p = 0.003). Infection by one or multiple HPV types was found in 11 out of 20 (55.0%) and 9 out of 20 (45.0%) samples, respectively. The evaluation of MN using cervical smears collected for cytology tests could, thus, be used as additional information to monitor a population's exposure to HPV.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572014005000010
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.37 n.2 2014
Fonte: | info:eu-repo/semantics/openAccess