Data/hora: 28/05/2024 17:34:48

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana	
Autores: 	Chalfun-Junior,Antonio; Mes,Jurriaan J.; Busscher,Marco; Angenent,Gerco C.	
Data: 	2006-01-01	
Ano: 	2006	
Palavras-chave: 	Arabidopsis; Development; Pod shattering; Transcription factors; Mutants.	
Resumo: 	Activation tagging is a powerful tool to identify new mutants and to obtain information about possible biological functions of the overexpressed genes. The quadruple cauliflower mosaic virus (CaMV) 35S enhancer fragment is a strong enhancer, which is most commonly used for this purpose. However, the constitutive nature of this enhancer may generate lethal mutations or aberrations in different plant organs by the same overexpressed gene. A tissue-specific activation tagging approach may overcome these drawbacks and may also lead more efficiently to the desired phenotype. For this reason the SHATTERPROOF2 (SHP2) promoter fragment was analysed for enhancer activity. The SHP2 gene is involved in dehiscence zone development and expressed during silique development. The aim of the experiments described here was to identify a dehiscence zone specific enhancer that could be used for tissue-specific activation tagging. The chosen SHP2 enhancer fragment was found to be expressed predominantly in the dehiscence zone and showed enhancer activity as well as ectopic expression activity. This activity was not influenced by its orientation towards the promoter and it was still functional at the largest tested distance of 2.0 kb. Based on these results, the SHP2 enhancer fragment can potentially be used in a tissue-specific activation tagging approach to identify new Arabidopsis mutants with an altered dehiscence zone formation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200032	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572006000200032	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.29 n.2 2006	
Fonte: 	info:eu-repo/semantics/openAccess