Data/hora: 05/06/2024 00:43:20

Provedor de dados: | 74
País: | Brazil
Título: | The effect of magnesium on mitotic spindle formation in Schizosaccharomyces pombe
Autores: | Uz,Gulsen; Sarikaya,Aysegul Topal.
Data: | 2016-09-01
Ano: | 2016
Palavras-chave: | Magnesium; Microtubule; Mitosis; Mitotic spindle; Schizosaccharomyces pombe.
Resumo: | Abstract Magnesium (Mg2+), an essential ion for cells and biological systems, is involved in a variety of cellular processes, including the formation and breakdown of microtubules. The results of a previous investigation suggested that as cells grow the intracellular Mg2+ concentration falls, thereby stimulating formation of the mitotic spindle. In the present work, we used a Mg2+-deficient Schizosaccharomyces pombe strain GA2, in which two essential membrane Mg2+ transporter genes (homologs of ALR1 and ALR2 in Saccharomyces cerevisae) were deleted, and its parental strain Sp292, to examine the extent to which low Mg2+ concentrations can affect mitotic spindle formation. The two S. pombe strains were transformed with a plasmid carrying a GFP-α2-tubulin construct to fluorescently label microtubules. Using the free Mg2+-specific fluorescent probe mag-fura-2, we confirmed that intracellular free Mg2+ levels were lower in GA2 than in the parental strain. Defects in interphase microtubule organization, a lower percentage of mitotic spindle formation and a reduced mitotic index were also observed in the GA2 strain. Although there was interphase microtubule polymerization, the lower level of mitotic spindle formation in the Mg2+-deficient strain suggested a greater requirement for Mg2+ in this phenomenon than previously thought.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300459
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-GMB-2015-0239
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.39 n.3 2016
Fonte: | info:eu-repo/semantics/openAccess