Data/hora: 09/06/2024 17:43:12

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
Autores: | Maluf,Mirian Perez; Silva,Carla Cristina da; Oliveira,Michelle de Paula Abreu de; Tavares,Aline Gomes; Silvarolla,Maria Bernadete; Guerreiro Filho,Oliveiro.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Coffee; Cup quality; Differential expression; Naturally decaffeinated.
Resumo: | In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400023
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009005000090
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess