Data/hora: 13/06/2024 02:42:49

Provedor de dados: | 74
País: | Brazil
Título: | Comparative transcriptome profile of the leaf elongation zone of wild barley (Hordeum spontaneum) eibi1 mutant and its isogenic wild type
Autores: | Zhou,Qin; Wang,Aidong; Duan,Ruijun; Yan,Jun; Zhao,Gang; Nevo,Eviatar; Chen,Guoxiong.
Data: | 2017-12-01
Ano: | 2017
Palavras-chave: | ABC transporter; Defense genes; Desiccation tolerance; Plant cuticle; RNA-Seq.
Resumo: | Abstract The naturally occurring wild barley mutant eibi1/hvabcg31 suffers from severe water loss due to the permeable leaf cuticle. Eibi1/HvABCG31 encodes a full ATP-binding cassette (ABC) transporter, HvABCG31, playing a role in cutin deposition in the elongation zone of growing barley leaves. The eibi1 allele has pleiotropic effects on the appearance of leaves, plant stature, fertility, spike and grain size, and rate of germination. Comparative transcriptome profile of the leaf elongation zone of the eibi1 mutant as well as its isogenic wild type showed that various pathogenesis-related genes were up-regulated in the eibi1 mutant. The known cuticle-related genes that we analyzed did not show significant expression difference between the mutant and wild type. These results suggest that the pleiotropic effects may be a compensatory consequence of the activation of defense genes in the eibi1 mutation. Furthermore, we were able to find the mutation of the eibi1/hvabcg31 allele by comparing transcript sequences, which indicated that the RNA-Seq is useful not only for researches on general molecular mechanism but also for the identification of possible mutant genes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500834
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2016-0321
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.40 n.4 2017
Fonte: | info:eu-repo/semantics/openAccess