Data/hora: 16/06/2024 05:02:23

Provedor de dados: | 74
País: | Brazil
Título: | Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens
Autores: | Manfroi,Ernandes; Yamazaki-Lau,Elene; Grando,Magali F.; Roesler,Eduardo A.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Co-cultivation conditions; Protocol establishment; GUS; T-DNA delivery.
Resumo: | Abstract Low transformation efficiency is one of the main limiting factors in the establishment of genetic transformation of wheat via Agrobacterium tumefaciens. To determine more favorable conditions for T-DNA delivery and explant regeneration after infection, this study investigated combinations of acetosyringone concentration and pH variation in the inoculation and co-cultivation media and co-culture temperatures using immature embryos from two Brazilian genotypes (BR 18 Terena and PF 020037). Based on transient expression of uidA, the most favorable conditions for T-DNA delivery were culture media with pH 5.0 and 5.4 combined with co-culture temperatures of 22 °C and 25 °C, and a 400 μM acetosyringone supplement. These conditions resulted in blue foci in 81% of the embryos. Media with more acidic pH also presented reduced A. tumefaciens overgrowth during co-culture, and improved regeneration frequency of the inoculated explants. BR 18 Terena was more susceptible to infection by A. tumefaciens than PF 020037. We found that it is possible to improve T-DNA delivery and explant regeneration by adjusting factors involved in the early stages of A. tumefaciens infection. This can contribute to establishing a stable transformation procedure in the future.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400470
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-475738420150026
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.38 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess