Data/hora: 09/06/2024 11:21:20

Provedor de dados: | 74
País: | Brazil
Título: | Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae)
Autores: | Carvalho,Vanda Marilza de; Marques,Rosimar Maria; Lapenta,Ana Sílvia; Machado,Maria de Fátima P.S.
Data: | 2003-01-01
Ano: | 2003
Palavras-chave: | Esterase classification; Peroba-rosa; Ops; Inhibition test.
Resumo: | Polyacrylamide gel electrophoresis system (PAGE) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases fromAspidosperma polyneuron. The characterization of alpha- and beta-esterases from young leaves of A. polyneuron by the PAGE system showed fourteen esterase isozymes. The differential staining pattern showed that Est-2 isozyme hydrolyzes beta-naphthyl acetate; Est-6, Est-7 and Est-8 isozymes hydrolyze alpha-naphthyl acetate, and Est-1, Est-3, Est-4, Est-5, Est-9, Est-10, Est-11, Est-12, Est-13, and Est-14 isozymes hydrolyze both alpha- and b-naphthyl acetate. Inhibition pattern of a- and beta-esterases showed that Folidol is a more potent inhibitor that Malathion, while Thiamethoxan (an insecticide with organophosphorus-like action) acts as an Est-4 and Est-6 inhibitor and induces the appearance of Est-5 and Est-7 isozymes as more intensely stained bands. Inhibition tests showed that OPC insecticides inhibit or activate plant esterases. Thus, plant esterases may be used as bioindicators to detect the presence and toxicity of residues of topically applied insecticides in agriculture and may be valuable for monitoring pollutants in the environment.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200013
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572003000200013
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.26 n.2 2003
Fonte: | info:eu-repo/semantics/openAccess