Data/hora: 04/06/2024 18:12:18

Provedor de dados: | 74
País: | Brazil
Título: | β-casein gene polymorphism permits identification of bovine milk mixed with bubaline milk in mozzarella cheese
Autores: | Otaviano,Antonio R.; Lima,André Luis F.; Laureano,Monyka M.M.; Sena,Janete A.D.; Albuquerque,Lúcia G. de; Tonhati,Humberto.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Casein; Gene polymorphism; Mozarella; Cheese authentication; PCR-RFLP.
Resumo: | Mozzarella cheese is traditionally prepared from bubaline (Bubalus bubalis) milk, but product adulteration occurs mainly by addition of or full substitution by bovine milk. The aim of this study was to show the usefulnes of molecular markers to identify the admixture of bovine milk to bubaline milk during the manufacturing process of mozzarella cheese. Samples of mozzarella cheese were produced by adding seven different concentrations of bovine milk: 0%, 1%, 2%, 5%, 8%, 12% and 100%. DNA extracted from somatic cells found in cheese were submitted to PCR-RFLP analysis of casein genes: α-s1-CN - CSN1S1 that encompasses 954 bp from exon VII to intron IX (Alu I and Hinf I), β-CN - CSN2 including 495 bp of exon VII (Hae III and Hinf I), and κ-CN - CSN3, encompassing 373 bp of exon IV (Alu I and Hind III). Our results indicate that Hae III-RFLP of CSN2 exon VII can be used as a molecular marker to detect the presence of bovine milk in "mozzarella" cheese.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500017
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572008005000002
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.31 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess