Data/hora: 10/06/2024 11:15:07

Provedor de dados: | 74
País: | Brazil
Título: | The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
Autores: | Mansouri,Vahid; Salehi,Mohammad; Nourozian,Mohsen; Fadaei,Fatemeh; Farahani,Reza Mastery; Piryaei,Abbas; Delbari,Ali.
Data: | 2015-06-01
Ano: | 2015
Palavras-chave: | Differentiation; Germ cells; Nuclear transfer embryonic stem cells.
Resumo: | Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of Mvh protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with Bmp4 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-475738138120140213
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.38 n.2 2015
Fonte: | info:eu-repo/semantics/openAccess