Data/hora: 02/06/2024 09:25:31

Provedor de dados: | 74
País: | Brazil
Título: | Chromosome mapping of ribosomal genes and histone H4 in the genus Radacridium (Romaleidae)
Autores: | Anjos,Allison; Loreto,Vilma; Souza,Maria José de.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA.
Resumo: | In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10 autosomes in R. nordestinum.In R. mariajoseae, the 5S rDNA sites were localized in the in L1 and L2 bivalents and in the X chromosome. In R. nordestinum, the 5S genes were located in the L2,L3,M4 and M5 pairs. In both species the histone H4 genes were present in a medium size bivalent. Together, these data evidence a great variability of chromosome markers and show that the 18S and 5S ribosomal genes are dispersed in the Radacridium genome without a significant correlation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572013005000026
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.36 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess