Data/hora: 10/06/2024 01:07:10

Provedor de dados: | 74
País: | Brazil
Título: | Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)
Autores: | Yaish,M.W.F.; Pérez de la Vega,M.
Data: | 2003-01-01
Ano: | 2003
Palavras-chave: | Phaseolus vulgaris L.; Genetic markers; Microsatellites; MADS-box; Transcriptional factor.
Resumo: | The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which represents the first MADS-box locus described to date in common bean, showed a very high similarity with other known MADS-box sequences and was grouped within the AGL2 subfamily cluster of the Arabidopsis MADS-box genes. The vicinity of microsatellites to some genes is also discussed.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300019
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572003000300019
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.26 n.3 2003
Fonte: | info:eu-repo/semantics/openAccess