Data/hora: 11/06/2024 15:06:41

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations	
Autores: 	Cestari,Marta Margarete; Lemos,Priscilla Maria M.; Ribeiro,Ciro Alberto de Oliveira; Costa,João Ricardo M. Alves; Pelletier,Emilien; Ferraro,Marcos V.M.; Mantovani,Mário Sérgio; Fenocchio,Alberto Sergio.	
Data: 	2004-01-01	
Ano: 	2004	
Palavras-chave: 	Chromosomal aberrations; Comet assay; Genotoxicity; Hoplias malabaricus; Lead; Mutagenesis.	
Resumo: 	The effects of clastogenic or mutagenic agents have rarely been studied in neotropical fish species exposed to contaminated water. In this study, the genetic damage caused by lead in the widely distributed South American fish, Hoplias malabaricus, was assessed using the comet (SCGE) assay and by testing for chromosomal aberrations. Eighteen specimens were acclimatized to laboratory conditions and then chronically exposed to contaminated food by feeding prey (Cyprinus sp.) injected intraperitoneally with doses of inorganic lead adjusted to give a contamination level of 21 mg of Pb2+.g-1 net weight of H. malabaricus. Three fish were sampled for chromosomal analysis after four doses (18 days) and another three after eight doses (41 days) of lead and the results then compared with three untreated controls kept under lead-free conditions. An additional six treated fish and three controls were sampled for the comet assay after 13 doses (64 days). Exposure to lead significantly increased the frequency of chromosomal aberrations and the frequency of tailed cell nuclei, the latter indicating DNA damage. These results show that H. malabaricus is a useful biological model for screening the clastogenic effects of lead and possibly other xenobiotics. The genetic damage seen here illustrates the need to investigate the potential effects of heavy metals on fish species in South America.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200023	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572004000200023	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.27 n.2 2004	
Fonte: 	info:eu-repo/semantics/openAccess