Data/hora: 10/06/2024 19:10:06

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
Autores: | Gerhardt,Liliane B. de A.; Magioli,Cláudia; Perez,Ana B.U.C.M.; Margis,Rogério; Sachetto-Martins,Gilberto; Margis-Pinheiro,Márcia.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Abiotic stress; Chia4 Arabidopsis thaliana endochitinase; Embryogenesis; Gene expression; Promoter.
Resumo: | The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of transcription. A negative regulatory region located between portions -1083 and -600 was also observed.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572004000100020
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.27 n.1 2004
Fonte: | info:eu-repo/semantics/openAccess