Data/hora: 04/06/2024 07:22:30

Provedor de dados: | 74
País: | Brazil
Título: | Individual sensitivity to cytogenetic effects of benzo[alpha]pyrene in cultured human lymphocytes: influence of glutathione S-transferase M1 genotype
Autores: | Güven,Gülgün S.; Güven,Mehmet; Onaran,Ilhan; Ulutin,Turgut; Hacihanefioglu,Seniha.
Data: | 2006-01-01
Ano: | 2006
Palavras-chave: | Sister chromatid exchanges; Chromosomal aberrations; Benzo[alpha]pyrene; Glutathione S-transferase M1.
Resumo: | Sister chromatid exchange (SCE) and chromosome aberrations (CA) in peripheral lymphocytes has been widely used in assessing exposure to mutagens and carcinogens. One of the extensively studied genotoxins is benzo[alpha]pyrene (BaP). We studied the ability of BaP to induce SCE and CA in 16 glutathione S-transferase M1 (GSTM1)-positive and 15 GSTM1-null individuals by analyzing 72-h whole-blood lymphocyte cultures, either BaP-untreated (controls) or treated with 5 µM of BaP for 24 or 48 h. There was no differences in the level of BaP-induced chromosomal aberrations between GSTM1-positive or null individuals when the cells were BaP-exposed for 24 h (0.083 ± 0.059 vs. 0.090 ± 0.058) or 48 h (0.092 ± 0.057 vs. 0.096 ± 0.050. The frequency of SCE in controls was GSTM1-positive = 2.96 ± 0.35 and GSTM1-null = 3.23 ± 0.56 while that for BaP-treated lymphocytes was GSTM1-positive = 5.56 ± 0.83 and GSTM1-null = 6.09 ± 1.11 and were not statistically significant. The rates of BaP-induced in vitro chromatid and chromosome-type gaps and breaks were similar in all groups, although GSTM1-null genotype chromatid-type breaks were more frequent (0.064 ± 0.039 per metaphase) than chromosome-type breaks (0.032 ± 0.027 per metaphase) after 48 h treatment with BaP (p &lt; 0.001). These findings suggest that BaP-induced in vitro SCE and CA are not influenced by the GSTM1 genotype.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100027
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572006000100027
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.29 n.1 2006
Fonte: | info:eu-repo/semantics/openAccess