Data/hora: 11/06/2024 06:30:41

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Clastogenic activity of 2-chlorodeoxyadenosine in mammalian somatic cells
Autores: | Antonucci,Gilmara Ausech; Takahashi,Catarina Satie.
Data: | 2005-01-01
Ano: | 2005
Palavras-chave: | Human lymphocytes; 2-CdA; Chromosomal aberrations; SCE; Cell cycle.
Resumo: | The base analogue 2-chlorodeoxyadenosine (2-CdA) used for therapy in chronic resistant and advanced lymphoproliferative disorders, is cytotoxic for both dividing and non-dividing lymphocytes. The present work evaluated the clastogenic potential of this drug in vitro in human lymphocytes in culture and in vivo in BALB/c mice bone marrow cells. In human lymphocytes, the clastogenic effect of 2-CdA was studied in G1, S and G2 phases of the cell cycle, using three different concentrations (10, 20 and 40 mug/mL). The endpoints analyzed included mitotic index (MI), proliferation index (PI), sister chromatid exchange (SCE), and chromosomal aberration (CA). Statistical analysis by a variance (ANOVA) test showed a significant increase (p &lt; 0.05) in CA frequencies for cells treated during the S phase, but the MI did not vary. The concentrations tested did not produce a significant increase in the mean frequency of SCEs, nor did they change the cell PI in the G1 and S phases. The concentrations in vivo tested were 0.25, 0.375 and 0.5 mg/kg body weight. In this assay, alterations in CA frequencies and MI were not observed at the dose levels tested. Therefore, the results indicate a clastogenic effect of 2-CdA in human lymphocyte cultures.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200027
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572005000200027
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.28 n.2 2005
Fonte: | info:eu-repo/semantics/openAccess