Data/hora: 13/06/2024 08:18:28

Provedor de dados: | 74
País: | Brazil
Título: | Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
Autores: | Torres-Silva,Carolina Furtado; Repolês,Bruno Marçal; Ornelas,Hugo Oliveira; Macedo,Andréa Mara; Franco,Glória Regina; Junho Pena,Sérgio Danilo; Tahara,Erich Birelli; Machado,Carlos Renato.
Data: | 2018-06-01
Ano: | 2018
Palavras-chave: | T. cruzi; DNA; Mutation frequency; H2O2.
Resumo: | Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic variability in this parasite. We developed an assay that employs a reporter gene that, once mutated in specific positions, convert G418-sensitive into G418-insenstitive T. cruzi. We were able to determine the frequency of DNA mutations in T. cruzi exposed and non-exposed to oxidative insults assessing the number of colony-forming units in solid selective media after plating a defined number of cells. We verified that T. cruzi‘s spontaneous mutation frequency was comparable to those found in other eukaryotes, and that exposure to hydrogen peroxide promoted a two-fold increase in T. cruzi‘s mutation frequency. We hypothesize that genetic mutations in T. cruzi can arise from oxidative insults faced by this parasite during its life cycle.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2017-0281
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.41 n.2 2018
Fonte: | info:eu-repo/semantics/openAccess