Data/hora: 10/06/2024 07:44:56

Provedor de dados: | 77
País: | Chile
Título: | Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
Autores: | Ili,Carmen G; Brebi,Priscilla; Garcia,Patricia; Leal,Pamela; Lopez,Jaime; Tapia,Oscar; Letelier,Pablo; Weber,Helga; Castillo,Jonathan; Roa,Juan C.
Data: | 2015-06-01
Ano: | 2015
Palavras-chave: | Apoptosis; Proliferation; Cervical cancer; SiRNA; C-FLIPL.
Resumo: | Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P&lt;0.05), while C-33A cells did not show significant differences. Ki-67 and PCNA immunocytochemistry was performed to evaluate proliferation on these cervical cancer cell lines. SiHa cells with c-FLIPL knockdown showed elevated expression of Ki-67 protein compared with their scrambled counterparts (P&lt;0.0001), while C-33A c-FLIPL knockdown cells showed a significantly lower in PCNA expression (P&lt;0.01) compared with control. All three c-FLIP-transfected cell lines showed a higher level of apoptosis compared with their scrambled controls. Our results suggest that c-FLIPL could have effects in proliferation and apoptosis in cervical cancer cell lines.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036
Editor: | Sociedad Chilena de Anatomía
Formato: | text/html
Fonte: | International Journal of Morphology  v.33 n.2 2015