Data/hora: 09/06/2024 05:48:17

Provedor de dados: | 79
País: | Brazil
Título: | POLYACRYLAMIDE GEL ELECTROPHORESIS AS A TOOL FOR THE TAXONOMIC IDENTIFICATION OF SNAKES FROM THE ELAPIDAE AND VIPERIDAE FAMILIES
Autores: | SOARES,A. M.; ANZALONI PEDROSA,L. H.; FONTES,M. R. M.; DA SILVA,R. J.; GIGLIO,J. R.
Data: | 1998-01-01
Ano: | 1998
Palavras-chave: | Snake venoms; Basic toxins; Polyacrylamide gel electrophoresis; Elapidae; Viperidae.
Resumo: | Polyacrylamide gel electrophoresis (PAGE) for basic proteins may be a useful tool for the characterization of whole snake venoms and for the taxonomic classification of snakes of the Elapidae and Viperidae families. However, due to the close proximity of molecular weights among toxins bearing different net charges, sodium dodecyl sulfate (SDS) - PAGE was not able to provide an efficient differentiation. This article reports the electrophoretic analysis of several venoms from the genera Micrurus, Bothrops, Bothriopsis, Crotalus and Lachesis and shows a typical and distinctive electrophoretic profile for each species, with intraspecific and geographic variations. Even in cases in which extreme morphological similarities were present, such as between B. jararacussu and B. pirajai ("Bahia jararacuçu"), differentiation could be evidenced by PAGE. This simple and sensitive procedure may be applied to similar cases involving basic toxins.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000200005
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Relação: | 10.1590/S0104-79301998000200005
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins  v.4 n.2 1998
Fonte: | info:eu-repo/semantics/openAccess