Data/hora: 09/06/2024 03:52:53

Provedor de dados: | 79
País: | Brazil
Título: | PRODUCTION OF AN EFFECTIVE ANTI-Bothrops-TETANUS MIXED HYPERIMMUNE SERUM OF EQUINE ORIGIN
Autores: | GUIDOLIN,R.; STEPHANO,M. A.; MORAIS,J. F.; SAKAGUTI,E. H.; PRADO,S. M. A.; FRATELLI,F.; VANCETTO,M. D. C.; HIGASHI,H. G.
Data: | 1998-01-01
Ano: | 1998
Palavras-chave: | Antivenoms; Immunization; Bothrops; Tetanus.
Resumo: | The present investigation reveals the possibility of simultaneous immunization of horses with Bothrops or Crotalus snake venoms and Tetanus antigens for the production of anti-Bothrops-Tetanus or anti-Crotalus-Tetanus mixed serum, with high titers of the respective specific antibodies. Bothrops antivenoms with an average neutralizing titer of 4.16 mg venom/ml were obtained from plasma of horses with titers lower than 0.5 mg venom/ml when Tetanus antigens were not used. This suggests the existence of a synergism between Bothrops venoms and Tetanus antigens in the stimulation of the antibody response. The pooled plasma of the animals had a neutralizing titer of 21.0 mg/ml reference Bothrops venoms and 3,300 IU/ml to Tetanus antigens after purification by enzymatic digestion and ammonium sulphate precipitation. These experiments lead us to conclude that Bothrops envenomation therapy can be successfully performed using anti-Bothrops-Tetanus serum also serving as Tetanus prophylaxis. Anti-Crotalus-Tetanus serum can also be produced, although it is not of medical interest as Crotalus envenomation rarely results in local necrotizing lesions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100007
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Relação: | 10.1590/S0104-79301998000100007
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins  v.4 n.1 1998
Fonte: | info:eu-repo/semantics/openAccess