Data/hora: 09/06/2024 06:26:41

Provedor de dados: 	Journal of Venomous Animals and Toxins	
País: 	Brazil	
Título: 	Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-I	
Autores: 	Oshima-Franco,Y.; Leite,G. B.; Valério,A. A.; Hyslop,S.; Andriao-Escarso,S.; Giglio,J. R.; Prado-Franceschi,J.; Cruz-Höfling,M. A.; Rodrigues-Simioni,L.	
Data: 	2002-01-01	
Ano: 	2002	
Palavras-chave: 	Bothrops venom; Myotoxin; Neutralization; Neuromuscular junction; Histological analysis; Bothrops jararacussu.	
Resumo: 	Bothrops jararacussu venom and its major toxin bothropstoxin-I (BthTX-I) possess myotoxic and neurotoxic properties. The efficacy of a rabbit antivenom raised against B. jararacussu venom in the neutralization of physiological, biochemical, and morphological changes induced by the venom and its major toxin BthTX-I was studied in mouse isolated phrenic nerve-diaphragm (PND) and extensor digitorum longus (EDL) preparations. The times required for 50% neuromuscular blockade in PND and EDL preparations for venom were 70+11.5 (S.E.M., n=5) min and 58+8 (n=16) (50 mu g/mL), and for BthTX-I 31+6 (n=3) min and 30+3 (n=5) min (20 mu g/mL), respectively. After 120 min incubation, creatine kinase (CK) concentrations in solution containing the EDL preparations were 3464+346 U/L after exposure to venom (50 mu g/mL, n=5) and 3422+135 U/L to BthTX-I (20mu g/mL, n=4), respectively. Rabbit antivenom dose-dependently neutralized venom and toxin-induced neuromuscular blockade in both preparations and effectively prevented venom and toxin-induced CK release from EDL. Histological analysis showed that rabbit antivenom neutralized morphological damage caused by B. jararacussu venom and BthTX-I in EDL preparations. These results indicate that rabbit antivenom effectively neutralized the biological activities of B. jararacussu venom and BthTX-I.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200004	
Editor: 	Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP	
Relação: 	10.1590/S0104-79302002000200004	
Formato: 	text/html	
Fonte: 	Journal of Venomous Animals and Toxins  v.8 n.2 2002	
Fonte: 	info:eu-repo/semantics/openAccess