Data/hora: 08/06/2024 00:30:30

Provedor de dados: | 80
País: | Brazil
Título: | rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro
Autores: | De Moraes,C. K.; Fritzen,M.; Chudzinski-Tavassi,A. M.; Selistre-de-Araújo,H. S.
Data: | 2008-01-01
Ano: | 2008
Palavras-chave: | RACLF; Snake venom metalloprotease; Endothelial cells; Inflammation.
Resumo: | Snake venom metalloproteases (SVMPs) comprise a family of snake venom toxins responsible for most of local and systemic effects observed during envenomation by snakes from the Viperidae family. The vascular system and more specifically the endothelium seem to be the preferential targets of these proteins. This work describes the effects of rACLF, a recombinant SVMP from Agkistrodon contortrix laticinctus on human umbilical vein endothelial cells (HUVECs) in vitro. Our results showed that rACLF activates HUVECs by the release of mediators involved in inflammation and hemostasis such as prostacyclin and interleukin-8. We also demonstrated that rACLF increased the expression of ICAM-I and decay accelerating factor (DAF). Moreover, rACLF protects the HUVECs against apoptosis induced by serum deprivation. These results suggest that the endothelial cell activation induced by SVMPs may have a significant role in the development of the local inflammatory lesion observed in Viperidae envenomation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100009
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992008000100009
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.14 n.1 2008
Fonte: | info:eu-repo/semantics/openAccess