Data/hora: 16/06/2024 11:09:20

Provedor de dados: | 80
País: | Brazil
Título: | IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: cross-reaction with venoms from Bothrops species from Argentina
Autores: | Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction.
Resumo: | We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992009000300008
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.15 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess