Data/hora: 08/06/2024 02:53:44

Provedor de dados: | Journal of Venomous Animals and Toxins Including Tropical Diseases
País: | Brazil
Título: | Biochemical and biological evaluation of gyroxin isolated from Crotalus durissus terrificus venom
Autores: | Barros,LC; Soares,AM; Costa,FL; Rodrigues,VM; Fuly,AL; Giglio,JR; Gallacci,M; Thomazini-Santos,IA; Barraviera,SRCS; Barraviera,B; Ferreira Junior,RS.
Data: | 2011-01-01
Ano: | 2011
Palavras-chave: | Gyroxin; Neurotoxicity; Coagulant activity; Crotalus durissus terrificus; Serine proteinase.
Resumo: | Gyroxin, a thrombin-like enzyme isolated from Crotalus durissus terrificus venom and capable of converting fibrinogen into fibrin, presents coagulant and neurotoxic activities. The aim of the present study was to evaluate such coagulant and toxic properties. Gyroxin was isolated using only two chromatographic steps - namely gel filtration (Sephadex G-75) and affinity (Benzamidine Sepharose 6B) - resulting in a sample of high purity, as evaluated by RP-HPLC C2/C18 and electrophoretic analysis that showed a molecular mass of 30 kDa. Gyroxin hydrolyzed specific chromogenic substrates, which caused it to be classified as a serine proteinase and thrombin-like enzyme. It was stable from pH 5.5 to 8.5 and inhibited by Mn²+, Cu²+, PMSF and benzamidine. Human plasma coagulation was more efficient at pH 6.0. An in vivo toxicity test showed that only behavioral alterations occurred, with no barrel rotation. Gyroxin was not able to block neuromuscular contraction in vitro, which suggests that its action, at the studied concentrations, has no effect on the peripheral nervous system.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100004
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992011000100004
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.17 n.1 2011
Fonte: | info:eu-repo/semantics/openAccess