Data/hora: 08/06/2024 01:05:27

Provedor de dados: 	Journal of Venomous Animals and Toxins Including Tropical Diseases	
País: 	Brazil	
Título: 	Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom	
Autores: 	Vinhote,JFC; Torres,AFC; Dantas,RT; Praciano,TP; Menezes,RRPPB; Sousa,DF; Brito,TS; Lima,FJB; Toyama,MH; Magalhães,PJ; Monteiro,HSA; Martins-Nunes,AMC.	
Data: 	2011-01-01	
Ano: 	2011	
Palavras-chave: 	Polybia paulista; Venom; Kidney; Aorta; MDCK.	
Resumo: 	In the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 μg/mL) had increased perfusion pressure, renal vascular resistance, urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of proteins in Bowman's space and tubular lumen, and focal areas of necrosis. The venom promoted a cytotoxic effect on Madin-Darby canine kidney (MDCK) cells. A significant increase in lactic dehydrogenase levels was observed in response to venom exposure. In isolated mesenteric vascular beds, pressure and vascular resistance augmented in a dose-dependent manner. PPV increased the contractility of aortic rings maintained under basal tension. This contractile response was inhibited when preparations were maintained in Ca2+-free medium. Likewise, verapamil, a voltage-gated calcium channel blocker, also inhibited the contractile response. In this study, phentolamine, a blocker of α-adrenergic receptor blocker, significantly reduced the contractile effect of PPV in the aortic ring. In conclusion, PPV produced nephrotoxicity, which suggests a direct effect on necrotic cellular death in renal tubule cells. The vascular contractile effect of PPV appears to involve calcium influx through voltage-gated calcium channels via adrenergic regulation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200011	
Editor: 	Centro de Estudos de Venenos e Animais Peçonhentos	
Relação: 	10.1590/S1678-91992011000200011	
Formato: 	text/html	
Fonte: 	Journal of Venomous Animals and Toxins including Tropical Diseases  v.17 n.2 2011	
Fonte: 	info:eu-repo/semantics/openAccess