Data/hora: 16/06/2024 06:53:06

Provedor de dados: | 82
País: | Brazil
Título: | Nucleopolyhedrovirus: scanning electron microscopy technique
Autores: | Torquato,Ednéia F.B.; Miranda Neto,Marcílio H. de; Brancalhão,Rose M.C.; Franco,Valdeni S.
Data: | 2006-12-01
Ano: | 2006
Palavras-chave: | Baculovirus; Bombyx mori; BmMNPV; Polyhedra; Truncated octahedron.
Resumo: | A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that identified it as a truncated octahedron. The form of the polyhedron can present taxonomic value, once it is specific for each viral lineage.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600011
Editor: | Sociedade Entomológica do Brasil
Relação: | 10.1590/S1519-566X2006000600011
Formato: | text/html
Fonte: | Neotropical Entomology  v.35 n.6 2006
Fonte: | info:eu-repo/semantics/openAccess