Data/hora: 12/06/2024 17:16:29

Provedor de dados: | Neotropical Entomology
País: | Brazil
Título: | Genetic differentiation in natural populations of Lutzomyia longipalpis (Lutz &amp;amp; Neiva) (Diptera: Psychodidae) with different phenotypic spot patterns on tergites in males
Autores: | Silva,MH; Nascimento,MDSB; Leonardo,FS; Rebêlo,JMM; Pereira,SRF.
Data: | 2011-08-01
Ano: | 2011
Palavras-chave: | Abdominal spot; Genetic polymorphism; Leishmaniasis; RAPD-PCR; Sand fly.
Resumo: | Entomological surveys in the state of Maranhão have recorded morphologically distinct populations of Lutzomyia longipalpis (Lutz &amp;amp; Neiva). Some populations have one pair of spots (1S) on the fourth tergite, while others have two pairs (2S) on the third and fourth tergites of males. In the present study we investigated the degree of genetic polymorphism among four populations in the municipalities of Caxias, Codó and Raposa, in the state of Maranhão, Brazil, by using RAPD (Random Amplified Polymorphic DNA) markers. A total of 35 loci were identified, of which 30 were polymorphic. The highest polymorphism was observed with primer OPA 4, which produced 11 different profiles. Genetic diversity was assessed using grouping methods that produced a dendrogram in which the genotypes could be clearly separated into two main clades according to the number of spots on the male abdominal tergites. One cluster contained the populations from Caxias and Codó, and the other was formed by the populations from Raposa and Codó. The results of our RAPD analysis showed a clear separation between the populations with one and two pairs of spots. The epidemiologic significance of this genetic differentiation should be investigated in future studies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000400015
Editor: | Sociedade Entomológica do Brasil
Relação: | 10.1590/S1519-566X2011000400015
Formato: | text/html
Fonte: | Neotropical Entomology  v.40 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess