Data/hora: 09/06/2024 18:48:30

Provedor de dados: | Neotropical Ichthyology
País: | Brazil
Título: | DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems
Autores: | Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando.
Data: | 2013-09-01
Ano: | 2013
Palavras-chave: | Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology.
Resumo: | In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the same species by some authors, showed 4.2% genetic divergence, reinforcing their are different species. Samples of A. affinis from the Uruguay and Paraguay rivers presented 0.3% genetic divergence, indicating a close relationship between them. However, these samples diverged 6.1% from the samples of the upper Paraná River, indicating that the latter represents a potentially new species. The results showed the effectiveness of the DNA barcoding method in identifying the analyzed species, which, together with the morphological and cytogenetic available data, help species identification.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497
Editor: | Sociedade Brasileira de Ictiologia
Relação: | 10.1590/S1679-62252013000300003
Formato: | text/html
Fonte: | Neotropical Ichthyology  v.11 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess