Data/hora: 09/06/2024 14:59:32

Provedor de dados: | Phyton: Revista Internacional de Botánica Experimental
País: | Argentina
Título: | Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis
Autores: | Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G.
Data: | 2012-06-01
Ano: | 2012
Palavras-chave: | Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes.
Resumo: | Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gamma­proteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they corresponded to 40% of the whole ribosomal library. Phylogenetic analysis showed that most of the ribosomal sequences are grouped into clades that belong to common rhizospheric or bulk-soil bacteria. To determine whether the sample is significantly diverse, a Shannon-Wiener test was performed, resulting in a rate of 3.8 bits per individual. Our results sug­gest that the rhizosphere of wheat plants is highly diverse and results an excellent candidate for metagenomic analysis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012
Editor: | Fundación Rómulo Raggio
Formato: | text/html
Fonte: | Phyton (Buenos Aires)  v.81 n.1 2012
Fonte: | info:eu-repo/semantics/openAccess