Data/hora: 03/06/2024 02:40:41

Provedor de dados: | 86
País: | Brazil
Título: | Paternity test in "Mangalarga-Marchador" equines by DNA-fingerprinting
Autores: | ANUNCIAÇÃO,CARLOS EDUARDO; ASTOLFI-FILHO,SPARTACO.
Data: | 2000-10-01
Ano: | 2000
Palavras-chave: | Breeding methods; Molecular cloning; Progeny testing; Horses; Identification; Genetic polymorphism.
Resumo: | GC-rich molecular minisatellite probes isolated from the human genome have presented a poor ability for individualization in horses. In this study new DNA sequences were isolated which could be used in paternity tests in horses. Genomic DNA from "Mangalarga-Marchador" horses was treated with restriction enzymes that preferentially digest non-repetitive sequences, so preserving the structure where mini and microsatellites are located. Four clones (S01, S05, S07 and S09) selected from a genomic library screened with a (TG)n oligonucleotide showed similar hybridization profiles generating bands of DNA-fingerprinting type. Using these probes the individualization power obtained was 10-8, which is 10(5)fold higher than that obtained with M13, another GC-rich type probe. All clones were efficient in parentage detection in crossbreedings and presented a 27 bp consensus sequence, GTTTCATTTATTATTCTTTGGAAGAAA, which was repeated 12, 18, 11 and 21 times in clones S01, S05, S07 and S09, respectively.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000001000012

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2000001000012
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.35 n.10 2000
Fonte: | info:eu-repo/semantics/openAccess