Data/hora: 09/06/2024 08:34:58

Provedor de dados: | 86
País: | Brazil
Título: | GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange
Autores: | Azevedo,Fernando Alves de; Mourão Filho,Francisco de Assis Alves; Schinor,Evandro Henrique; Paoli,Luis Gustavo de; Mendes,Beatriz Madalena Januzzi; Harakava,Ricardo; Gabriel,Dean W.; Lee,Richard F.
Data: | 2006-11-01
Ano: | 2006
Palavras-chave: | Xylella fastidiosa; PAL; Citrus variegated chlorosis; Cultivar resistance.
Resumo: | The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100008

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2006001100008
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.41 n.11 2006
Fonte: | info:eu-repo/semantics/openAccess