Data/hora: 09/06/2024 05:25:59

Provedor de dados: | 86
País: | Brazil
Título: | Glutenin analysis for the safe screening of self-pollinated wheat seeds when developing doubled-haploid populations
Autores: | Torres,Gisele Abigail Montan; Scagliusi,Sandra Maria Mansur; Chaves,Márcia Soares; Consoli,Luciano.
Data: | 2018-05-01
Ano: | 2018
Palavras-chave: | Puccinia recondita f. sp. tritici; Triticum aestivum; Adult plant resistance; Leaf rust; Microspore culture; Toropi wheat cultivar.
Resumo: | Abstract: The objective of this work was to apply glutenin analysis, as a simple and reliable method, to identify self-pollinated wheat (Triticum aestivum) seeds in F1 crosses, when developing mapping populations using doubled-haploid (DH) technology. F1 seeds were subjected to glutenin analyses using gel electrophoresis, to verify and confirm their heterozygotic profiles. Glutenin analyses were carried out using one half of the endosperm of each of the 87 F1 seeds. The other half of the endosperm was used to develop the mapping population through DHs. Plants with band patterns different from the expected ones were discarded. Although the incidence of self-pollinated seeds was relatively low (only 8%), the screening of F1 heterozygotic profiles is extremely important, particularly when creating reliable mapping populations.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500646

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/s0100-204x2018000500014
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.53 n.5 2018
Fonte: | info:eu-repo/semantics/openAccess