Data/hora: 01/06/2024 18:00:00

Provedor de dados: | Pesquisa Agropecuária Brasileira
País: | Brazil
Título: | Tannin-tolerant bacteria from crossbred Holstein x Zebu cows
Autores: | Arcuri,Pedro Braga; Odenyo,Agnes Awino; Arcuri,Edna Froeder; Ribeiro,Marlice Teixeira; Guimarães,Marta Fonseca Martins; Carneiro,Jailton da Costa.
Data: | 2011-03-01
Ano: | 2011
Palavras-chave: | Butyrivibrio fibrisolvens; Streptococcus bovis; Anti-nutritional polyphenolics; Ruminant nutrition.
Resumo: | The objective of this work was to isolate and characterize tannin-tolerant ruminal bacteria from crossbred Holstein x Zebu cows fed a chopped mixture of elephant grass (Pennisetum purpureum), young stems of "angico-vermelho" (Parapiptadenia rigida), and banana tree (Musa sp.) leaves. A total of 117 bacteria strains were isolated from enrichment cultures of rumen microflora in medium containing tannin extracts. Of these, 11 isolates were able to tolerate up to 3 g L-1 of tannins. Classical characterization procedures indicated that different morphological and physiological groups were represented. Restriction fragments profiles using Alu1 and Taq1 of 1,450 bp PCR products from the 16S rRNA gene grouped the 11 isolates into types I to VI. Sequencing of 16S rRNA PCR products was used for identification. From the 11 strains studied, seven were not identifiable by the methods used in this work, two were strains of Butyrivibrio fibrisolvens, and two of Streptococcus bovis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000300007

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2011000300007
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.46 n.3 2011
Fonte: | info:eu-repo/semantics/openAccess