Data/hora: 09/06/2024 13:46:02

Provedor de dados: | 86
País: | Brazil
Título: | In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
Autores: | Silva,Tatiane Loureiro da; Scherwinski-Pereira,Jonny Everson.
Data: | 2011-04-01
Ano: | 2011
Palavras-chave: | Ex situ conservation; Germplasm bank; Micropropagation.
Resumo: | The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400007

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2011000400007
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.46 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess