Data/hora: 12/06/2024 14:06:40

Provedor de dados: | 86
País: | Brazil
Título: | Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
Autores: | Nogueira,Gabriela Ferreira; Pasqual,Moacir; Scherwinski-Pereira,Jonny Everson.
Data: | 2013-11-01
Ano: | 2013
Palavras-chave: | Saccharum; In vitro conservation; PVS2; Regeneration.
Resumo: | The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013001100014

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2013001100014
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.48 n.11 2013
Fonte: | info:eu-repo/semantics/openAccess