Data/hora: 10/06/2024 19:13:59

Provedor de dados: | 86
País: | Brazil
Título: | Evolutionary dynamics of Cucurbita argyrosperma from the Mesoamerican domestication center using SSR molecular markers
Autores: | Priori,Daniela; Villarreal,Daniel Zizumbo; Ek,Víctor Manuel de Jesús Canché; Limones-Briones,Verónica; Barbieri,Rosa Lía.
Data: | 2018-03-01
Ano: | 2018
Palavras-chave: | Cucurbitaceae; Genetic diversity; Microsatellite markers; Plant genetic resources; Squash.
Resumo: | Abstract: The objective of this work was to evaluate the evolutionary dynamics of the wild-weedy-domestic gene pool of Cucurbita argyrosperma squash by estimating the levels of genetic diversity and gene flow in the putative area of its domestication. Nine populations were collected, and DNA was extracted from young leaves harvested separately from approximately 20 individuals in each population. The DNA fragments were amplified with eight pairs of SSR primers and separated by electrophoresis in 5% denaturing polyacrylamide gels. Genetic diversity and the amount of gene flow were estimated in the populations, and Bayesian grouping was used to determine the levels of gene infiltration and probability of ancestry. The ethnobotanical exploration indicated that the evolutionary dynamics in the area occurred under five different ecological scenarios. Eighty-seven alleles with 75% to 100% polymorphic loci were identified. The greater genetic diversity in the weedy-domestic populations may have been the product of recombination due to the high gene flow between these populations promoted by pollinators and human selection. There is high gene flow between the wild and cultivated populations of C. argyrosperma in its domestication centre, highlighting the importance of conserving and maintaining these genetic resources.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300287

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/s0100-204x2018000300003
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.53 n.3 2018
Fonte: | info:eu-repo/semantics/openAccess