Data/hora: 02/06/2024 07:26:07

Provedor de dados: | 86
País: | Brazil
Título: | Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil
Autores: | Lazarotto,Marília; Bovolini,Marciéli Pitorini; Muniz,Marlove Fátima Brião; Harakawa,Ricardo; Reiniger,Lia Rejane Silveira; Santos,Álvaro Figueredo dos.
Data: | 2014-06-01
Ano: | 2014
Palavras-chave: | Carya illinoinensis; ?-tubulin; ITS rDNA; Morphological characters; Phylogeny; UPGMA.
Resumo: | The objective of this work was to characterize and cluster isolates of Pestalotiopsis species and to identify those that are pathogenic to pecan, based on morphological and molecular characters. Pestalotiopsis spp. isolates were identified by sequencing the internal transcribed spacer (ITS) and β?tubulin regions. Identification methods were compared to indicate the key morphological characters for species characterization. Thirteen isolates were used for the pathogenicity tests. Morphological characterization was performed using the following variables: mycelial growth rate, sporulation, colony pigmentation, and conidial length and width. Ten pathogenic isolates were identified, three as -tubulin regions. Identification methods were compared to indicate the key morphological characters for species characterization. Thirteen isolates were used for the pathogenicity tests. Morphological characterization was performed using the following variables: mycelial growth rate, sporulation, colony pigmentation, and conidial length and width. Ten pathogenic isolates were identified, three as Pestalotiopsis clavispora and three as P. cocculi. The other isolates remained as an undefined species. The morphological characters were efficient for an initial separation of the isolates, which were grouped according to differences at species level, mainly colony diameter, which was identified as an important morphological describer. Beta-tubulin gene sequencing was less informative than the ITS region sequencing for species identification.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014000600440

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2014000600005
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.49 n.6 2014
Fonte: | info:eu-repo/semantics/openAccess