Data/hora: 02/06/2024 01:23:30

Provedor de dados: | 86
País: | Brazil
Título: | In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
Autores: | Sihler,William; Souza,Marlinda Lobo de; Valicente,Fernando Hercos; Falcão,Rosana; Sanches,Marcio Martinello.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Baculovirus; Biological control; Cell culture; Fall armyworm; In vitro production; SfMNPV.
Resumo: | Abstract: The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDS-Page of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100001

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/s0100-204x2018000100001
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.53 n.1 2018
Fonte: | info:eu-repo/semantics/openAccess