Data/hora: 09/06/2024 10:46:18

Provedor de dados: | 86
País: | Brazil
Título: | Embryo rescue from interspecific crosses in apple rootstocks
Autores: | Dantas,Adriana Cibele de Mesquita; Boneti,José Itamar; Nodari,Rubens Onofre; Guerra,Miguel Pedro.
Data: | 2006-06-01
Ano: | 2006
Palavras-chave: | Malus; Marubakaido; M9; Culture medium; Carbon source.
Resumo: | The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from hand-pollination, cultivated in medium supplemented with 14 µM IAA, 5 µM kinetin and 1.5 µM Ga3 (MS1), mainly those of M. prunifolia x M. pumila, showed a high percentage of rusted embryos (96.2%). Embryos from open pollination of M. prunifolia and M. pumila formed calluses. It was possible to identify the influence of the female parent by the enhanced development of M. pumila shoots derived from open or hand-pollination. The crossing of responsive species and the use of the technique of embryo culture provided a rapid and uniform germination and, consequently, the development of fully normal seedlings.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600011

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2006000600011
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.41 n.6 2006
Fonte: | info:eu-repo/semantics/openAccess