Data/hora: 09/06/2024 13:51:12

Provedor de dados: | 86
País: | Brazil
Título: | Marker-assisted pyramiding of resistance loci to grape downy mildew
Autores: | Saifert,Luciano; Sánchez-Mora,Fernando David; Assumpção,Wilson Taybar; Zanghelini,Jean Alberto; Giacometti,Renan; Novak,Eduardo Irineu; Vesco,Lírio Luiz Dal; Nodari,Rubens Onofre; Eibach,Rudolf; Welter,Leocir José.
Data: | 2018-05-01
Ano: | 2018
Palavras-chave: | Plasmopara viticola; Vitis vinifera; Enological quality; Genetic resistance to disease; Grapevine breeding; Quantitative trait loci.
Resumo: | Abstract: The objective of this work was to use a marker-assisted selection for pyramiding the resistance loci Rpv1 and Rpv3.1 in grapevine (Vitis vinifera), and to evaluate their conferred resistance against Brazilian downy mildew (Plasmopara viticola) isolates. A progeny of 23 plants, segregating for the two resistance loci, was obtained by the cross between the Gf 2000-305-122 and Gf.Ga-52-42 genotypes. The progeny was genotyped with four microsatellite markers and phenotyped for resistance to P. viticola using a bioassay with leaf discs. Six plants containing the Rpv1 and Rpv3.1 pyramided loci were identified by the molecular analysis. Plants harboring the Rpv1 + Rpv3.1, Rpv3.1, and Rpv1 loci showed 12.8, 30.0, and 33.1 sporangiophores per leaf disc, respectively. Plants with no resistance loci showed a dense sporulation. The phenotypic analysis of the expression of the two pyramided loci was only confirmed for four plants that showed the highest resistance level, i.e., mean value of 1.8 sporangiophores. A high-throughput method for pyramiding the Rpv1 and Rpv3.1 loci was developed, which confirmed the increased resistance to P. viticola. The selected elite genetic material shows a high resistance to downy mildew and elevated enological potential for grapevine breeding in Brazil.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500602

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/s0100-204x2018000500009
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.53 n.5 2018
Fonte: | info:eu-repo/semantics/openAccess